You can set it up according to your general specifications (e.g. The core of the program is based around its workspace (Figure 1) and this is where you add all your samples, create various gates and statistics, and set up groups, etc. Analyzing Your Data with FlowjoĮssentially FlowJo is solely analysis software, though it does run acquisition on reconditioned cytometers by Cytek Development (“ FlowJo Collectors’ Edition”). We are starting this series off with FlowJo, which has become very popular, in particular among many in the immunology world who may have large complex datasets.
In this series of articles we are going to be looking at a range of software that is available to you for your cytometric analysis and give you the low down on each one, highlighting the strengths and weaknesses so you can make the decision of which software is best for you. So where we use the dedicated software aligned to the analyser for acquisition, we frequently use alternative programs for offline analysis.Īdvantages may be that such software is much more user-friendly for large scale analysis rather than the instrument-linked software (although there are usually analysis tools here too), and it can unify the analysis approach and presentation, so irrespective of what user or cytometer, the data “looks” the same.īut with the variety of software available how do you know which one is right for you? This can be increasingly complex depending on the number of cells, populations, parameters, and combinations. Generally, once you’ve acquired your samples on a cytometer, the hard (but hopefully fun!) part of making sense of all the data begins.
0 kommentar(er)
